Isolation of Ganoderma lucidum (Curtis) P. Karst. From the Wild in Lagos through Tissue Culture Techniques and Cultivation on Sawdust of Six Nigerian Hardwoods

Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used cultivation Asia, America Europe are available The present study investigated indigenous hardwoods their supplementation with rice wheat bran as substrates its cultivation. Six (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were potential growth potentially improve biological efficiency. was collected from the wild identification confirmed by amplifying ribosomal DNA-ITS fragment ITS1 ITS4 primers. Tissue culture initiated successfully modified malt extract agar grain spawn developed it. incubated after inoculation at room temperature 30-60 days. Substrates given cold treatment 7days to induce fructification which achieved 9-10h/day regime daily watering. There full mycelial ramification all mushroom. Fruit bodies harvested but alata. highest yield (308.76±5.81g/kg) recorded Mansonia (Biological Efficiency-31.42±4.55%) least (31.45±5.44g/kg) (B.E- 5.25±0.58%). performed significantly better than supplemented substrates. Commercial native G. Nigeria possible local agricultural wastes.